InhaltsangabePart I: Introduction to C. difficile and the Disease It Causes 1. Clostridium difficile: No Longer an Enigmatic Pathogen? Adam P. Roberts and Peter Mullany 2. Clostridium difficile and the Disease It Causes Torbjörn Norén Part II: Isolation and Culture Techniques 3. Clostridium difficile Isolation and Culture Techniques Mike Wren Part III: Methods for Typing and Epidemiological Studies 4. Molecular Typing Methods for C. difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping Sandra Janezic and Maja Rupnik 5. Clostridium difficile Toxinotyping Maja Rupnik 6. Multilocus Sequence Typing for Clostridium difficile Ludovic Lemée and Jean-Louis Pons Part IV: Biochemistry of the Organism 7. Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes Ana Antunes and Bruno Dupuy 8. Dissecting the Cell Surface Robert Fagan and Neil Fairweather 9. Human Intestinal Epithelial Response(s) to Clostridium difficile Nazila V. Jafari, Elaine Allan, and Mona Bajaj-Elliott Part V: Genomics 10. Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays Richard Stabler, Lisa Dawson, and Brendan Wren Part VI: Development of Systems for Genetic Analysis of the Organism 11. ClosTronTargeted Mutagenesis John T. Heap, Stephen T. Cartman, Sarah A. Kuehne, Clare Cooksley, and Nigel P. Minton 12. Methods for Gene Cloning and Targeted Mutagenesis Glen P. Carter, Dena Lyras, Rachael Poon, Pauline M. Howarth, and Julian I. Rood 13. Transposon Mutagenesis in C. difficile Haitham A. Hussain, Adam P. Roberts, Rachael Whalan, and Peter Mullany Part VII: Animal Models of Disease 14. Refinement of the Hamster Model of Clostridium difficile Disease Gillian Douce and David Gouding 15. Methods for Working with the Mouse Model Anne Collignon