InhaltsangabePrefaceContributorsI.                   Introduction1.      Fungal Transformation: From Protoplasts to Targeted Recombination SystemsJuan F. MartínII.                Transformation Methods: Protoplast Transformation2.      Protoplast Transformation for Genome Manipulation in FungiAroa Rodriguez-Iglesias and Monika Schmoll3.      Trichoderma Transformation MethodsMónica G. Malmierca, Rosa E. Cardoza and Santiago Gutiérrez4.      Transformation of Mucor circinelloides f. lusitanicus Protoplasts Victoriano Garre, José Luis Barredo and Enrique A. Iturriaga5.      Transformation of Saccharomyces cerevisiae: Spheroplast MethodShigeyuki Kawai and Kousaku MurataIII.             Transformation Methods: Electroporation6.      Electroporation Mediated DNA Transformation of Filamentous FungiB.N. Chakraborty7.      Chemical Transformation of Candida albicans Sophie Bachellier-Bassi and Christophe d'Enfert8.      Electroporation of Pichia pastoris Knut Madden, Ilya Tolstorukov and James Cregg9.      Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) Kostyantyn Dmytruk and Andriy SibirnyIV.             Transformation Methods: Particle Bombardment10.  Biolistic Transformation for Delivering DNA into the MitochondriaArianna Montanari, Monique Bolotin-Fukuhara, Mario Fazzi D'Orsi, Cristina De Luca, Michele M. Bianchi and Silvia Francisci11.  Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome TransformantsJingwen Zhou, Liming Liu, Guocheng Du and Jian Chen12.  Use of the Biolistic Particle Delivery System to Transform Fungal GenomesV. S. Junior Te'o and K. M. Helena Nevalainen13.  Transformation of Zygomycete Mortierella alpina Using Biolistic Particle BombardmentEiji Sakuradani, Hiroshi Kikukawa, Seiki Takeno, Akinori Ando, Sakayu Shimizu and Jun OgawaV.              Transformation Methods: Agrobacterium-Mediated Transformation14.  Agrobacterium tumefaciens-Mediated Transformation Rasmus John Normand Frandsen15.  Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red YeastsGiuseppe Ianiri and Alexander Idnurm16.  Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporumManish Pareek, Mahak Sachdev, Meenakshi Tetorya and Manchikatla V. RajamVI.             Transformation Methods: Li-acetate Transformation17.  High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG MethodR. Daniel Gietz18.  Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying MechanismShigeyuki Kawai and Kousaku Murata19.  Transformation of Lithium Acetate-treated Neurospora crassaJohn V. PaiettaVII.          Transformation Methods: New developments20.  Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast CellsYevhen Filyak, Nataliya Finiuk, Nataliya Mitina, Alexander Zaichenko and Rostyslav Stoika21.  Transformation of Fungi Using Shock WavesMiguel A. Gómez-Lim, Denis Magaña Ortíz, Francisco Fernández and Achim M. LoskeVIII.       Exogenous DNA: Uptake of DNA22.  Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast GeneticsPetar Tomev Mitrikeski23.  Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell CycleAurelijus Zimkus, Audrius Misiunas, Arunas Ramanavicius and Larisa ChaustovaIX.             Exogenous DNA: Integration of DNA24.  Recombination

Marco A. van den Berg, PhDPrincipal Scientist Applied Biochemistry and ScreeningDSM Biotechnology CenterDelft, The Netherlands Karunakaran Maruthachalam, PhDCurrent: Research ScientistGlobal Marker Technology Lab, DuPont-PioneerDuPont Knowledge CenterAndhra Pradesh, India Previous: Molecular BiologistCalifornia Seed and Plant LabsPleasant Grove, CA, USA