InhaltsangabePart I. Introduction and Overview  1. Protein-Ligand Interactions: FundamentalsMark A. Williams 2. Protein Sample CharacterizationTina Daviter and Rémi Fronzes 3. Measurement of Protein-Ligand Complex FormationPeter N. Lowe, Cara Vaughan, and Tina Daviter Part II. Quantitation of Thermodynamics and Kinetics  4. Isothermal Titration Calorimetry for Studying Protein-Ligand InteractionsLuminita Damian 5. Rapid Mixing Kinetic TechniquesStephen R. Martin and Maria J. Schilstra 6. Protein-Ligand Interactions Using SPR SystemsÅsa Frostell, Lena Vinterbäck and Hans Sjöbom Part III. Spectroscopic Methods 7. Fluorescence Techniques in Analysis of Protein-Ligand Interactions        Gabor Mocz and Justin A. Ross 8. Circular and Linear Dichroism Spectroscopy for the Study of Protein-Ligand InteractionsTina Daviter, Nikola Chmel, and Alison Rodger 9. Analyzing Protein-Ligand Interactions by Dynamic NMR SpectroscopyAnthony Mittermaier and Erick Meneses 10. Studying Metal Ion - Protein Interactions: Electronic Absorption, Circular Dichroism and Electron Paramagnetic ResonanceLilliana Quintanar and Lina Rivillas-Acevedo 11. Monitoring Protein-Ligand Interactions by Time-resolved FTIR Difference SpectroscopyCarsten Kötting and Klaus Gerwert Part IV. Ligand Discovery  12. Biophysical Methods in Drug Discovery from Small Molecule to PharmaceuticalGeoffrey Holdgate, Stefan Geschwindner, Alex Breeze, Gareth Davies, Nicola Colclough, David Temesi, and Lara Ward 13. Biophysical Screening for the Discovery of Small-Molecule LigandsAlessio Ciulli 14. Screening Protein-Small Molecule Interactions by NMRBen Davis Part V. Molecules in Cellular Environments  15. Model Membrane SystemsHeiko Keller, Remigiusz Worch, and Petra Schwille 16. Quantitative Fluorescence Co-localization to Study Protein-Receptor ComplexesShanica N. Pompey, Peter Michaely, and Katherine Luby-Phelps Part VI. Structural & Computational Methods 17. Studying Protein-Ligand Interactions Using X-Ray CrystallographyAndrew P. Turnbull and Paul Emsley 18. Molecular Fields in Ligand DiscoveryPaul J. Gane and A. W. Edith Chan 19. Structure-Based Virtual Screening for Novel LigandsWilliam R. Pitt, Mark D. Calmiano, Boris Kroeplien, Richard D. Taylor, James P. Turner, and Michael A. King